Cyanocobalamin (Vit B12)
Nme:Cyanocobalamin (Vit B12)
CAS:68-19-9
MF:C63H88CoN14O14P
The detailed specifications are as below:
CAS:68-19-9
MF:C63H88CoN14O14P
The detailed specifications are as below:
| Item of testing | Specification | Result | |
| Appearance | Dark-red crystalline powder or crystals | conforms | |
| Ratio of optical density | A361/A550nm | 3.15 ~ 3.40 | 3.28 |
| A361/A278nm | 1.70 ~ 1.88 | 1.86 | |
| TLC | conforms | conforms | |
| Chemical | positive | positive | |
| HPLC | The retention time of major peak of the sample solution corresponds to that of the standard solution | Correspond | |
| Related substances | |||
| Total impurities,% | ≦3.0% | 1.6% | |
| Single impurities | |||
| 7β,8β-lactocyanocobalamin,% | ≦1.0% | 0.3% | |
| 50-carboxycyanocobalamin,% | ≦0.5% | Not detected | |
| 34-methylcyanocobalamin,% | ≦2.0% | 0.5% | |
| 32-carboxycyanocobalamin | ≦1.0% | Not detected | |
| 8-Epi-cyanocobalamin | ≦1.0% | Not detected | |
| Item of testing | Specification | Result |
| Any other unidentified impurity,% | ≦0.5% | 0.3% |
| Loss on drying,% | ≦12.0% | 2.2% |
| Assay on dried basis,% | 96.0~102.0% | 99.0% |
| Residual solvents | ||
| Acetone,ppm | ≦1000ppm | 16ppm |
| Ethanol,ppm | ≦1000ppm | 2.1×102ppm |
| Conclusion | This product complied with the specifications of USP36 and Ph.Eur.7.0 | |